Pseudostaurosira brevistriata can be confused with other ellipticial, araphid species. Larger specimens of P. brevistrata are lanceolate in valve outline, but smaller specimens may not be distinguishable from P. elliptica in LM. Pseudostaurosira neoelliptica has round areolae and hollow spines. Although Staurosira construens var. venter also possesses short striae, the striae are narrower and composed of smaller areolae than those of P. brevistriata.